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KMID : 0614020090240010068
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Journal of Pharmaceutical Sciences (C.N.U.)
2009 Volume.24 No. 1 p.68 ~ p.76
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Protective Effects of Catechol on tert-Butly hydroperoxide Induced Oxidative Hepatotoxicity
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Shin Dong-Hee
Nam Jin-Kyung
Park Chang-Sik
Ko Ji-Hun
Myung Pyung-Keun
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Abstract
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This study was to evaluate the protective effects of the catechol compound of Camellia japonica leaf on the tert-butyl hydroperoxide(t-BHP) induced oxidative injury in mice. To examine the in vitro effects of catechol on oxidative stress, human hepatoma cell line, Hep G2 was used and the tetrazolium dye colorimetric test(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay was employed to monitor cytotoxicity. The in vivo study was carried out by administering catechol(0.01, 0.1, 1mg/kg b.w.) by orally for 5 days before a single dose of t-BHP(1.5 mmol/kg i.p.). When the concentration of catechol pretreatment was increased, the liver MDA level was decreased. It was shown that catechol treatment did not alter the serum levels of the hepatic enzyme markers such as aspartate amino-transferase(AST) and alanine aminotransferase(ALT). Next, expression of antioxidant enzymes in liver after catechol treatment were studied using RT-PCR. The liver mRNA expression for antioxidant enzymes; such as Cu/Zn SOD or extracellular SOD were increased after catechol treatment group. These results suggest that catechol may have a potential role in the prevention of oxidative damage of liver in living organism.
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KEYWORD
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Oxidative stress, MTT assay, liver MDA, SOD
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